2017 NIH SCAP-T: Immune Cell Diversity

(Stephen Fisher) #1

Thurs, June 29th, Breakout Session

Moderator: Y. William Lu, Ph.D. (National Cancer Institute)

Immunologists usually rely on flow cytometry and other traditional tools to conduct immune-related research. In the past several years, new single-cell technologies, such as single-cell transcriptomics and mass cytometry (CyTOF), have enabled researchers to ask scientific questions that could not be addressed previously. The theme for this session is to discuss the opportunities and potential challenges in immune-related studies, such as cell type diversity and phenotypic analysis.

Questions for this breakout session to consider include:

  • What immunological questions can we ask from the CyTOF analysis?
  • Similarly, what immunological questions can we ask from the single-cell transcriptome analysis?
  • Several technologies are available for single-cell transcriptome analysis. What are the pros and cons for these technologies? Which technology is suitable for immune-related studies?
  • What kind of data quality should we expect in order to generate reproducible results? Should we validate the data by additional assays?
  • One of the potential challenges is the communication between scientists with different expertise. Sometimes the experiment may not perform well as expected. As an immunologist, a molecular biologist or a bioinformatician, what is the challenges to communicate with scientists with other disciplines?
  • What kind of community resources can we establish to help immunologists to understand and utilize new single-cell technologies?

(Pawel Osmulski) #2

Beyond transcriptome: ability to test how single cells (immune cells) interact with each other. Not high throughput yet but AFM could be helpful to answer a question how strong is cells attraction and deadly it could be.